Search results for " Influenza Virus"

showing 7 items of 7 documents

Traditional Chinese herbal medicine at the forefront battle against COVID-19: Clinical experience and scientific basis.

2020

Abstract Background Throughout the 5000-year history of China, more than 300 epidemics were recorded. Traditional Chinese herbal medicine (TCM) has been used effectively to combat each of these epidemics’ infections, and saved many lives. To date, there are hundreds of herbal TCM formulae developed for the purpose of prevention and treatment during epidemic infections. When COVID-19 ravaged the Wuhan district in China in early January 2020, without a deep understanding about the nature of COVID-19, patients admitted to the TCM Hospital in Wuhan were immediately treated with TCM and reported later with >90% efficacy. Approach We conducted conduct a systematic survey of various TCM herbal pre…

PTGS2 Prostaglandin-endoperoxide synthase 2BattleAIV avian influenza virusCoV coronavirusPharmaceutical ScienceiNOS nitric oxide synthaseViral infection0302 clinical medicinePA patchouli alcoholSARS Severe Acute Respiratory SyndromeSMD Sheganmahuang decoctionDrug DiscoveryPandemicIL InterleukinMedicine Chinese TraditionalALI acute lung injuriesmedia_commonCOVID-19 coronavirus disease 2019MXSG Ma xing shi gan decoction0303 health sciencesTNF tumor necrosis factorClinical Trials as TopicCCL2 CC chemokine ligand 2FM1 FM1 coronavirusICU intensive care unitc-AMP cyclic adenosine phosphateHIV human immunodeficiency virus030220 oncology & carcinogenesisCOX-2 cyclooxygenase-2Molecular MedicineHerbal preparationsMedicinal herbsAbbreviations: ACE2 angiotensin-converting enzyme IITCM traditional Chinese medicineHSV-1 herpes simplex virus 1CASP3 caspase 3medicine.medical_specialtyChinaCoronavirus disease 2019 (COVID-19)Systematic surveymedia_common.quotation_subjectJEV Japanese encephalitis virusNF-κB nuclear factor kappa B cellsAntiviral AgentsArticleWHO World Health Organization03 medical and health sciencesIEC-6 rat intestinal epithelial cell line 6SOD superoxide dismutaseCDC Center for Disease Control and PreventionmedicineAVP arginine vasopressinPGE2 prostaglandin E2HumansIntensive care medicineLH Lianhuaqingwen capsule030304 developmental biologyPharmacologyMedicinal herbMDA malondialdehydeGCGJ Gancao ganjiang decoctionNO nitric oxidePlants Medicinalbusiness.industrySARS-CoV-2COVID-19CXCL C-X-C- motif chemokineMDCK Madin-Darby Canine Kidney cellsTLR-4 Toll-like receptor-4COVID-19 Drug TreatmentComplementary and alternative medicineViral infectionLPS lipopolysaccharidesRSV respiratory syncytial virusQFPD Qingfeipaidu decoctionbusinessLung congestionECMO extracorporeal membrane oxygenationMAPK mitogen-activated protein kinasePhytotherapyDrugs Chinese HerbalPhytomedicine : international journal of phytotherapy and phytopharmacology
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Direct Visualization of the Conformational Dynamics of Single Influenza Hemagglutinin Trimers

2018

Influenza hemagglutinin (HA) is the canonical type I viral envelope glycoprotein and provides a template for the membrane-fusion mechanisms of numerous viruses. The current model of HA-mediated membrane fusion describes a static "spring-loaded" fusion domain (HA2) at neutral pH. Acidic pH triggers a singular irreversible conformational rearrangement in HA2 that fuses viral and cellular membranes. Here, using single-molecule Förster resonance energy transfer (smFRET)-imaging, we directly visualized pH-triggered conformational changes of HA trimers on the viral surface. Our analyses reveal reversible exchange between the pre-fusion and two intermediate conformations of HA2. Acidification of p…

0301 basic medicineProtein ConformationHemagglutinin (influenza)Hemagglutinin Glycoproteins Influenza VirusBiologyArticleGeneral Biochemistry Genetics and Molecular BiologyReaction coordinate03 medical and health sciencesViral envelopeInfluenza HumanFluorescence Resonance Energy TransferHumansDynamic equilibriumFusionCell MembraneLipid bilayer fusionHydrogen-Ion ConcentrationVirus InternalizationSingle Molecule ImagingHEK293 CellsHemagglutinins030104 developmental biologyMembraneFörster resonance energy transferA549 CellsInfluenza A virusBiophysicsbiology.proteinProtein BindingCell
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Transmission of hemagglutinin D222G mutant strain of pandemic (H1N1) 2009 virus.

2010

A pandemic (H1N1) 2009 virus strain carrying the D222G mutation was identified in a severely ill man and was transmitted to a household contact. Only mild illness developed in the contact, despite his obesity and diabetes. The isolated virus reacted fully with an antiserum against the pandemic vaccine strain.

MaleEpidemiologyvirusesMutantResistancelcsh:MedicineHemagglutinin Glycoproteins Influenza VirusSettore MED/42 - Igiene Generale E Applicatamedicine.disease_causeSeverity of Illness IndexDisease Outbreakschemistry.chemical_compoundInfluenza A Virus H1N1 SubtypePandemicInfluenza A virusA/H1N1PhylogenyTransmission (medicine)H1N1DispatchtransmissionMiddle AgedInfectious DiseasesD222GItalyInfluenza A virusRNA ViralinfluenzaMicrobiology (medical)AdultOseltamivirMutation MissenseHemagglutinin (influenza)BiologyViruslcsh:Infectious and parasitic diseasesMicrobiologyOseltamivirInfluenza HumanmedicineHumanslcsh:RC109-216virusesRetrospective StudiesAntiserumSequence Analysis RNApandemiclcsh:RMutantVirologyInfluenzaH1N1 subtypechemistryAmino Acid Substitutionbiology.proteinA/H1N1vmutationInfluenza; A/H1N1v; Oseltamivir; ResistanceEmerging infectious diseases
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A Trans-amplifying RNA Vaccine Strategy for Induction of Potent Protective Immunity

2019

Here, we present a potent RNA vaccine approach based on a novel bipartite vector system using trans-amplifying RNA (taRNA). The vector cassette encoding the vaccine antigen originates from an alphaviral self-amplifying RNA (saRNA), from which the replicase was deleted to form a transreplicon. Replicase activity is provided in trans by a second molecule, either by a standard saRNA or an optimized non-replicating mRNA (nrRNA). The latter delivered 10- to 100-fold higher transreplicon expression than the former. Moreover, expression driven by the nrRNA-encoded replicase in the taRNA system was as efficient as in a conventional monopartite saRNA system. We show that the superiority of nrRNA- ov…

Translational efficiencyGenetic VectorsRNA-dependent RNA polymeraseHemagglutinin (influenza)Hemagglutinin Glycoproteins Influenza VirusBiologyAntibodies ViralMadin Darby Canine Kidney CellsMice03 medical and health sciencesDogsImmunogenicity VaccineInfluenza A Virus H1N1 Subtype0302 clinical medicineOrthomyxoviridae InfectionsCricetinaeInfluenza HumanDrug DiscoveryGeneticsAnimalsHumansViral Replicase Complex ProteinsRepliconMolecular BiologyGene030304 developmental biologyPharmacologyMice Inbred BALB C0303 health sciencesMessenger RNAVaccinationRNATranslation (biology)Antibodies NeutralizingSemliki forest virusVirologyHEK293 CellsInfluenza Vaccines030220 oncology & carcinogenesisbiology.proteinRNA ViralMolecular MedicineFemaleOriginal ArticleMolecular Therapy
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Genotyping of a nosocomial outbreak of pandemic influenza A/H1N1 2009

2011

Background: Epidemiological surveys have revealed outbreaks of pandemic influenza A (H1N1) 2009 in several different contexts. Molecular characterization of the influenza virus could help to provide a more accurate description of these outbreaks. Objective: To genotype pandemic influenza A (H1N1) 2009 isolates from an epidemiologically defined nosocomial outbreak. Study design: We sequenced the neuraminidase (NA) and hemagglutinin (HA) influenza A (H1N1) 2009 genes from ten HIV-positive patients involved in an epidemiologically defined outbreak in the Clinical Microbiology and Infectious Diseases (CMID) Department. Sequences were aligned to search for specific genetic features of the involv…

Genotyping TechniquesGenotypeMolecular Sequence DataNeuraminidaseHemagglutinin Glycoproteins Influenza VirusContext (language use)medicine.disease_causePandemic H1N1Disease OutbreaksViral ProteinsInfluenza A Virus H1N1 SubtypeVirologyPandemicHIV SeropositivityInfluenza HumanInfluenza A virusmedicineHumansViral ProteinSequencingHemagglutinin Glycoproteins Influenza ViruPandemicsGenotypingPhylogenyCross InfectionDisease OutbreakbiologyPandemicCoinfectionTransmissibilityOutbreakvirus diseasesOutbreakVirologyInfluenza A virus subtype H5N1Infectious DiseasesAmino Acid SubstitutionMutationbiology.proteinHuman mortality from H5N1Genotyping TechniqueNeuraminidaseHuman
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The molecular epidemiology and evolutionary dynamics of influenza B virus in two Italian regions during 2010-2015: The experience of Sicily and Ligur…

2016

Molecular epidemiology of influenza B virus remained poorly studied in Italy, despite representing a major contributor to seasonal epidemics. This study aimed to reconstruct the phylogenetic relationships and genetic diversity of the hemagglutinin gene sequences of 197 influenza B strains circulating in both Southern (Sicily) and Northern (Liguria) Italy between 2010 and 2015. Upper respiratory tract specimens of patients displaying symptoms of influenza-like illness were screened by real-time RT-PCR assay for the presence of influenza B virus. PCR-positive influenza B samples were further analyzed by sequencing. Neighbor-joining phylogenetic trees were constructed and the amino-acid alignm…

0301 basic medicineInfluenza ViruslineagesHemagglutinin Glycoproteins Influenza VirusLiguriaSettore MED/42 - Igiene Generale E Applicatamolecular epidemiologyCatalysilcsh:Chemistryviral evolutionCladeinfluenza BSicilylcsh:QH301-705.5PhylogenySpectroscopyPhylogenetic treeinfluenza B; lineages; viral evolution; surveillance; molecular epidemiology; influenza-like illness; Sicily; Liguria; ItalyComputer Science Applications1707 Computer Vision and Pattern RecognitionGeneral MedicineBiological EvolutionComputer Science ApplicationsInfluenza B; Influenza-like illness; Italy; Liguria; Lineages; Molecular epidemiology; Sicily; Surveillance; Viral evolution; Biological Evolution; Genetic Variation; Hemagglutinin Glycoproteins Influenza Virus; Humans; Influenza B virus; Influenza Human; Italy; Molecular Epidemiology; Sicily; Phylogeny; Catalysis; Molecular Biology; Computer Science Applications1707 Computer Vision and Pattern Recognition; Spectroscopy; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic ChemistryInfluenza B; Influenza-like illness; Italy; Liguria; Lineages; Molecular epidemiology; Sicily; Surveillance; Viral evolution; Catalysis; Molecular Biology; Spectroscopy; Computer Science Applications1707 Computer Vision and Pattern Recognition; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic ChemistryItalyViral evolutionsurveillanceHumanHemagglutinin Glycoproteinsinfluenza-like illnessHemagglutinin (influenza)BiologyArticleCatalysisVirusInorganic Chemistry03 medical and health sciencesLineagePhylogeneticsInfluenza HumanHumansPhysical and Theoretical ChemistryMolecular BiologyInfluenza-like illneInfluenza-like illnessMolecular epidemiologyOrganic ChemistryGenetic VariationVirologyInfluenzaInfluenza B virus030104 developmental biologylcsh:Biology (General)lcsh:QD1-999biology.protein
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Genetic characterization of influenza viruses from influenza-related hospital admissions in the St. Petersburg and Valencia sites of the Global Influ…

2016

Abstract Background Continuous surveillance for genetic changes in circulating influenza viruses is needed to guide influenza prevention and control. Objectives To compare intra-seasonal influenza genetic diversity of hemagglutinin in influenza A strains isolated from influenza hospital admissions collected at two distinct sites during the same season. Study design Comparative phylogenetic analysis of full-length hemagglutinin genes from 77 isolated influenza A viruses from the St. Petersburg, Russian Federation and Valencia, Spain sites of the Global Influenza Hospital Surveillance Network (GIHSN) during the 2013/14 season. Results We found significant variability in A(H3N2) and A(H1N1)pdm…

0301 basic medicineHemagglutinin glycoproteinsMalevirusesHemagglutinin Glycoproteins Influenza Virusmedicine.disease_causeGlobal HealthRussia0302 clinical medicineGenomic sequencingInfluenza A Virus H1N1 SubtypeInfluenza A virusH3N2 subtype030212 general & internal medicineCladeChildPhylogenyAged 80 and overPhylogenetic treevirus diseasesMiddle AgedInfectious DiseasesInfluenza A virusChild PreschoolEpidemiological MonitoringRNA ViralFemaleSeasonsAdultAdolescentHemagglutinin (influenza)Genome ViralBiologyH5N1 genetic structure03 medical and health sciencesYoung AdultVirologyInfluenza preventionGenetic variationInfluenza HumanmedicineHumansAgedGenetic diversityInfluenza A Virus H3N2 SubtypeGenetic VariationInfantSequence Analysis DNAVirology030104 developmental biologyH1N1 subtypeSpainbiology.proteinInfluenza virusJournal of Clinical Virology
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